Dujardin and Valegro were the only pair to score over 80 per cent.
The trio, team gold medallists at the European championships, filled the top four places along with Richard Davison who already has qualifiers under his belt and stands eighth in the Reem Acra rankings.
Dujardin and Valegro – a horse co-owned by her trainer and mentor Hester – posted a score of 81.04 per cent to land the £2,700 top prize on her Olympia debut.
Bechtolsheimer and Mistral Hojris finished second on 79.23 per cent, while Hester and Uthopia took third with 75.44 per cent.
The London International Horse Show's dressage programme continues with the World Cup freestyle to music competition on Wednesday before showjumping takes centre-stage from Thursday, culminating in next Sunday's Rolex World Cup class that was won last year by Britain's Michael Whitaker.